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Sanger Sequencing

 
 

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Quanti-IT for Fluorometric RNA Measurements Print E-mail

About RNA Fluorometry

 

The Quant-IT RNA Reagent is a highly sensitive fluorescent dye that binds to RNA, and is highly selective for RNA over double-stranded DNA. We use this dye in conjuction with a fluorometer to measure the concentration of DNA in your sample. The nucleic acid specificity makes this assay more accurate than measurements based on spectrophotometry, such as those obtained from a Nanodrop or similar instrument. The spectrophotometric measurements will detect any components of your sample that absorb at the same wavelength as RNA - such 'contaminants' include PCR primers, nucleotides, DNA, phenol (sometimes used to remove protein from DNA preparations), thiocyanates (component of many commercial 'clean up' kits) and many others. It also allows the measurement of much smaller amounts of RNA - down to 250pg/uL


Using the Service


 
To use the Quant-IT RNA Fluorometry service, simply log your samples in iLab, our online sample submission system. Once your samples are logged, you can deliver your samples at any time during business hours. We will first estimate the concentration (using spectrophotometry) to help us determine the appropriate assay to use. We will then measure your sample fluorometrically and prepare a report containing the relevant data. This will be emailed to you using the details you included on the Sample Submission Form.

Log in to iLab to submit your sample details (existing users)

Monday, 12 March 2012 03:01